Uji Kepekaan terhadap Antibiotik dan Kemampuan Membentuk Biofilm pada Escherichia Coli Penyebab Infeksi Saluran Kemih
Keywords:
biofilm, escherichia coli, kepekaan antibiotik
Abstract
Escherichia coli merupakan bakteri yang sering menyebabkan infeksi saluran kemih (ISK) dan memiliki kemampuan membentuk biofilm di dalam jaringan, sehingga mengakhibatkan pengobatan infeksi menjadi lebih sulit. Tujuan Penelitian ini adalah mengetahui kepekaan E. coli yang mengakibatkan infeksi saluran kemih terhadap beberapa antibiotik dan kemampuannya dalam membentuk biofilm. Penelitian ini didahului dengan identifikasi E. coli yang diisolasi dari sampel urin penderita ISK dengan metode uji biokimia dan uji kepekaan berbagai antibiotik dengan metode dilusi (vitek 2 Compact). Uji kemampuan pembentukan biofilm dilakukan dengan metode Microtiter Plate Culture pada media Trypticase broth, diinkubasi selama 24 jam dengan suhu 37oC. Biofilm yang terbentuk diukur berdasarkan masa yang melekat pada sumuran micotiter plate 96 well dengan pewarnaan crystal violet 0,1%, dibaca dengan microplate reader pada panjang gelombang 570nm, dengan masing-masing ulangan 12 kali. Pengukuran biofilm berupa nilai Optical density dikategorikan dalam kuat, sedang, dan lemah. Hasil penelitian menunjukan semua isolat E. coli memiliki kesamaan sifat biokimia, namun memiliki kepekaan yang bervariasi terhadap beberapa antibiotik. Ada 14% isolat memiliki kemampuan pembentukan biofilm kuat, dan 43% isolat sedang, dan 43% isolat lemah, tidak ditemukan hubungan antara pembentukan biofilm dan kepekaannya terhadap antibiotik.
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Axler-DiPerte, G. L. (2017). Modeling and Visualizing Bacterial Colony Purification Without the Use of Bacteria or Laboratory Equipment. Journal of Microbiology & Biology Education, 18(2). https://doi.org/10.1128/jmbe.v18i2.1308
Cepas, V., López, Y., Muñoz, E., Rolo, D., Ardanuy, C., Martí, S., Xercavins, M., Horcajada, J. P., Bosch, J., & Soto, S. M. (2019). Relationship between Biofilm Formation and Antimicrobial Resistance in Gram-Negative Bacteria. Microbial Drug Resistance, 25(1), 72–79. https://doi.org/10.1089/mdr.2018.0027
Chu, E. K., Kilic, O., Cho, H., Groisman, A., & Levchenko, A. (2018). Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-06552-z
Drusano, G. L., Neely, M. N., Yamada, W. M., Duncanson, B., Brown, D., Maynard, M., Vicchiarelli, M., & Louie, A. (2018). The Combination of fosfomycin plus meropenem is synergistic for pseudomonas aeruginosa PAO1 in a hollow-fiber infection model. Antimicrobial Agents and Chemotherapy, 62(12), 1–9. https://doi.org/10.1128/AAC.01682-18
Endriani, R., Andrini, F., & Alfina, D. (2012). Pola Resistensi Bakteri Penyebab Infeksi Saluran Kemih (ISK) Terhadap Antibakteri di Pekanbaru. Jurnal Natur Indonesia, 12(2), 130. https://doi.org/10.31258/jnat.12.2.130-135
Fang, K., Jin, X., & Hong, S. H. (2018). Probiotic Escherichia coli inhibits biofilm formation of pathogenic E. coli via extracellular activity of DegP. Scientific Reports, 8(1), 1–12. https://doi.org/10.1038/s41598-018-23180-1
Frieri, M., Kumar, K., & Boutin, A. (2017). Antibiotic resistance. Journal of Infection and Public Health, 10(4), 369–378. https://doi.org/10.1016/j.jiph.2016.08.007
Hassan, A., Usman, J., Kaleem, F., Omair, M., Khalid, A., & Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. Brazilian Journal of Infectious Diseases, 15(4), 305–311. https://doi.org/10.1590/S1413-86702011000400002
Karigoudar, R. M., Karigoudar, M. H., Wavare, S. M., & Mangalgi, S. S. (2019). Detection of biofilm among uropathogenic Escherichia coli and its correlation with antibiotic resistance pattern. Journal of Laboratory Physicians, 11(01), 017–022. https://doi.org/10.4103/jlp.jlp_98_18
Karjono, B.-J., Susilaningsih, N., & Damma Purnawati, R. (2009). Pola kuman pada penderita Infeksi Saluran Kemihdi RSUP Dr Kariadi Semarang Microbiology pattern of urinary tract infection in Dr Kariadi General Hospital Semarang. Jurnal Kedokteran Yarsi, 17(2), 119–124.
Laganenka, L. (2018). crossm Autoinducer 2-Dependent Escherichia coli Biofilm Formation Is. American Society for Microbiology, 84(5), 1–15. https://www-ncbi-nlm-nih-gov.proxy-ub.rug.nl/pmc/articles/PMC5812939/pdf/e02638-17.pdf
Laverty, G., Gorman, S. P., & Gilmore, B. F. (2014). Biomolecular mechanisms of Pseudomonas aeruginosa and Escherichia coli biofilm formation. Pathogens, 3(3), 596–632. https://doi.org/10.3390/pathogens3030596
LeCuyer, T. E., Byrne, B. A., Daniels, J. B., Diaz-Campos, D. V., Hammac, G. K., Miller, C. B., Besser, T. E., & Davis, M. A. (2018). Population structure and antimicrobial resistance of canine uropathogenic Escherichia coli. Journal of Clinical Microbiology, 56(9). https://doi.org/10.1128/JCM.00788-18
Mirani, Z. A., Fatima, A., Urooj, S., Aziz, M., Khan, M. N., & Abbas, T. (2018). Relationshipof cell surfacehydrophobicitywithbiofilmformationand growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Iranian Journal of Basic Medical Sciences, 21(7), 760–769. https://doi.org/10.22038/ijbms.2018.28525.6917
Mulcahy, L. R., Burns, J. L., Lory, S., & Lewis, K. (2010). Emergence of Pseudomonas aeruginosa strains producing high levels of persister cells in patients with cystic fibrosis. Journal of Bacteriology, 192(23), 6191–6199. https://doi.org/10.1128/JB.01651-09
Naber, K., Bergman, B., & Bishop, M. (2001). Guidelines on the Management of Urinary and Male Genital Tract Infections Guidelines on the Management of Uti. 3(March).
Nair, S., Gomez-Cruz, J., Manjarrez-Hernandez, Á., Ascanio, G., Sabat, R. G., & Escobedo, C. (2018). Selective uropathogenic e. Coli detection using crossed surface-relief gratings. Sensors (Switzerland), 18(11), 1–12. https://doi.org/10.3390/s18113634
Nermine, E., Said, A., Gehan, E., Asmaa, H., & Asmaa, Y. (2019). Virulence Genes in Pseudomonas Aeruginosa Strains Isolated at Suez Canal University Hospitals with Respect to the Site of Infection and Antimicrobial Resistance. International Journal of Clinical Microbiology and Biochemical Technology, 2(1), 008–019. https://doi.org/10.29328/journal.ijcmbt.1001006
Sahputri, J., Lindarto, D., & Ganie, R. A. (2018). Negatif Dengan Resistensi Antibiotik Pada Wanita Diabetes Melitus Tipe 2 Relationship Between the Formation of Biofilm By Gram Negative. 4(1).
Shah, S. R., Tatara, A. M., D’Souza, R. N., Mikos, A. G., & Kasper, F. K. (2013). Evolving strategies for preventing biofilm on implantable materials. Materials Today, 16(5), 177–182. https://doi.org/10.1016/j.mattod.2013.05.003
Wahyudi, D., Aman, A. T., Handayani, N. S. N., & Soetarto, E. S. (2019). Differences among clinical isolates of Pseudomonas aeruginosa in their capability of forming biofilms and their susceptibility to antibiotics. Biodiversitas, 20(5), 1450–1456. https://doi.org/10.13057/biodiv/d200538
Wahyudi, D., & Wael, S. (2021). Pengaruh Ekstrak Alpinia galanga L Terhadap Produksi Biofilm pada Escherichia coli. Jurnal Farmasi (Journal of Pharmacy), 10(1), 24–30. https://doi.org/10.37013/jf.v10i1.117
Wang, L., Di Luca, M., Tkhilaishvili, T., Trampuz, A., & Gonzalez Moreno, M. (2019). Synergistic Activity of Fosfomycin, Ciprofloxacin, and Gentamicin Against Escherichia coli and Pseudomonas aeruginosa Biofilms. Frontiers in Microbiology, 10(November). https://doi.org/10.3389/fmicb.2019.02522
Published
2021-11-28
How to Cite
Wahyudi, D., & Rahmawati, A. (2021). Uji Kepekaan terhadap Antibiotik dan Kemampuan Membentuk Biofilm pada Escherichia Coli Penyebab Infeksi Saluran Kemih. Jurnal Farmasetis, 10(2), 83-94. https://doi.org/https://doi.org/10.32583/farmasetis.v10i2.1679
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Articles
